Nutrient Broth

ARTICLE NO. OLFC-1265
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For the general cultivation of less fastidious microorganisms, can be enriched with blood.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder.
Color Beige colored powder.
Solubility Soluble in Distilled / de-ionized water at 50℃
Color Pale yellow colored clear to slightly opalescent solution
pH 7.4 ±0.2 at 25℃ (13.00 g/ l)

Contents

Ingredients Gram/Litre
Peptone5.0
Meat Extract1.5
Yeast Extract1.5
Sodium Chloride5.0
pH at 25˚C7.4 ±0.2


Directions

Dissolve 13.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.