For detection and enumeration of coliform bacteria in water, waste water, dairy products &
other foods samples.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light amber colored clear opalescent solution
pH
6.8 ±0.2 at 25 ˚C (35.60 g/ l)
Contents
Ingredients
Gram/Litre
Tryptose
20.000
Lactose
5.000
Potassium Phosphate Dibasic
2.750
Potassium Phosphate Monobasic
2.750
Sodium Chloride
5.000
Sodium Lauryl Sulfate
0.100
pH at 25˚C
6.8 ±0.2
Directions
Dissolve 35.60 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute in test tube containing inverted Durham’s tube. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
Note: for 1.0 or less than 1.0 ml of water sample single strength broth is used and for more than 1.0 ml double strength broth is used.
