For the cultivation of less fastidious microorganisms, can be enriched with blood or other
biological fluids
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Pale yellow colored opalescent solution
pH
7.4 ±0.2 at 25 ˚C (28.00 g/ l)
Solidification
40 ˚C
Color
Pale yellow colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
5.0
Meat extract
1.5
Yeast Extract
1.5
Sodium chloride
5.0
Agar
15.0
pH at 25˚C
7.4±0.2
Directions
Dissolve 28.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in desired. Ensure complete solidification and inoculate test sample aseptically.
