For the isolation and differentiation between various species of Bacillus by chromogenic method
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Red color clear, opalescent solution
pH
7.20 ±0.2 at 25 ˚C (50.00 g/ l)
Solidification
40˚C
Contents
Ingredients
Gram/Litre
Special Peptone
10.000
Meat extract
1.000
D-Mannitol
10.000
Sodium Chloride
10.000
Chromogenic mixture
3.200
Phenol red
0.025
Agar
15.000
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 50.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.
Mix well and pour into sterile petri plates. To increase selectivity add polymyxin B (50000 units/
1000 ml of medium)
