For the detection and enumeration of Escherichia coli in foods without further confirmation on
membrane filter or by indole reagent
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent solution
pH
7.20 ±0.2 at 25℃ (36.60 g/ l)
Solidification
40˚C
Color
Light amber, clear opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
14.000
Peptone
5.000
Sodium chloride
2.400
Bile Salts
1.500
Disodium hydrogen phosphate
1.000
Sodium dihydrogen phosphate
0.600
X-glucoronide
0.075
Agar
12.000
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 36.60 grams (the equivalent weight of dehydrated medium per liter) in 1000 ml distilled
water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121 ℃, at 15 psi for 15 min; cool to 45-50°C. Mix well and distribute aseptically in petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.
