Recommended for the isolation and identification of Cronobacter sakazakii from food product
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent solution
pH
7.30 ±0.2 at 25˚C (32.40 g/ l)
Solidification
40˚C
Color
Light amber, clear opalescent gel
Contents
Ingredients
Gram/Litre
Tryptic digest of casein
7.000
Yeast extract
3.000
Sodium thiosulfate
1.000
Sodium deoxycholate
0.250
Sodium chloride
5.000
Ferric ammonium citrate
1.000
Chromogenic mixture
0.150
Agar
15.000
pH at 25˚C
7.3 ±0.2
Directions
Dissolve 32.40 grams in 1000 ml distilled water. Sterilize the media by autoclaving at 15lbs
pressure and 121℃ for 15 minutes. Cool to 45-50°C and distribute aseptically in petri plates, allow to
solidify. Ensure complete solidification and inoculate test sample aseptically.
