For the selective isolation of ESBL producing organisms
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent solution
pH
6.80±0.2 at 25˚C (47.50 g/ l)
Solidification
40˚C
Color
Light beige, clear opalescent gel
Contents
Ingredients
Gram/Litre
Special Peptone
12.00
Chromogenic Mixture
4.00
Sodium Chloride
5.00
Phosphate buffering
4.00
Agar
15.00
pH at 25˚C
6.8 ±0.2
Directions
Dissolve 40.00 grams in 990 ml distilled water. Boil to dissolve the medium completely.
Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Add two vials of
rehydrated content of ESBL selective supplement or 10 ml of antibiotic solution contain Ceftazidime (3.0 mg), Cefotaxime (3.0 mg), Ceftraizone (2.0 mg), Aztreonam (2.0 mg) and fluconazole (10.0 mg). Mix well and pour into sterile petri plates.
