For the enumeration of Escherichia coli from food samples, animal feed and water samples
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent
pH
7.2 ±0.2 at 25 ˚C (39.60 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
20.000
Bile Salt
1.500
X-Β-D-Glucuronic Acid
0.075
Dimethyl sulfoxide
3.000
Agar
15.000
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 39.60 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
