For selective isolation and differentiation of Vibrio species
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light yellow color, clear to slightly opalescent gel
pH
8.50 ± 0.2 at 25℃ (67.50 g/ l)
Solidification
40˚C
Contents
Ingredients
Gram/Litre
Peptone
10.000
Yeast Extract
3.000
Sodium Thiosulphate
5.000
Sodium Citrate
6.000
Sodium Cholate
1.000
Sodium Chloride
25.000
Chromogenic mixture
2.5000
Agar
15.000
pH at 25˚C
8.5±0.2
Directions
Dissolve 67.50 grams in 1000 ml distilled water. Heat to boiling to dissolve the
medium completely. DO NOT AUTOCLAVE. DO NOT OVERHEAT. Cool to 45-50°C. Mix well
and distribute aseptically in petri plates and allow to solidify. Ensure complete solidification
inoculate test sample aseptically
