Optimized formulation for the growth and maintenance of M13 phage or other
filamentous ss DNA bacteriophages.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Pale yellow colored opalescent
pH
7.0 ±0.2 at 25 ˚C (46.00 g/ l)
Solidification
40˚C
Color
Cream yellow colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
16.000
Yeast Extract
10.000
Sodium Chloride
5.000
Agar
15.000
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 46.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically. Ensure complete solidification and inoculate test sample aseptically.
