For cultivation & isolation of microorganisms encountered in breweries & industrial fermentations
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light green colored
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light bluish with greenish tinge, opalescent gel
pH
5.5 ±0.2 at 25 ˚C (80.25 g/ l)
Solidification
40˚C
Contents
Ingredients
Gram/Litre
Tryptone
5.000
Yeast Extract
4.000
Dextrose
50.000
Potassium dihydrogen phosphate
0.550
Potassium chloride
0.425
Calcium chloride
0.125
Ferric chloride
0.0025
Magnesium sulfate
0.0025
Manganese sulfate
0.125
Bromocresol green
0.022
Agar
20.000
pH at 25˚C
5.5±0.2
Directions
Dissolve 80.25 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in desired. Ensure complete solidification and inoculate test sample
aseptically.
Note: To obtaining bakers and distillers yeast counts adjust the pH 6.5 by using 1% sodium bicarbonate.
