For isolation and cultivation of Salmonella typhi with addition of selective reagent.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent
pH
7.3 ±0.2 at 25 ˚C (60.00 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel. After addition of bismuth ammonium Sulphate reagent and 4 ml of
Contents
Ingredients
Gram/Litre
Meat Extract
5.00
Peptone
10.00
Dextrose
10.00
Sodium chloride
5.00
Agar
30.00
pH at 25˚C
7.3 ±0.2
Directions
Dissolve 60.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely. Sterilize the medium by autoclaving at 15 lbs pressure at 121˚C for 15 minutes; cool
it to 42-45 °C. Add 70 ml of bismuth ammonium sulphite reagent and 4 ml of 1% brilliant green
solution. Mix well and distribute aseptically in petri plates with gentile shaking for equal
distribution. Ensure complete solidification and inoculate test sample aseptically.
