For the differentiation between Bacillus cereus and Bacillus subtilis based on mannitol
fermentation.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Greenish beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Basal medium: Light purple colored
pH
7.2 ±0.2 at 25 ˚C (22.00 g/L)
Solidification
40˚C
Color
Light purple colored clear to slightly opalescent gel.
Contents
Ingredients
Gram/Litre
Yeast autolysate
0.200
Mannitol
5.000
Monohydrogen ammonium phosphate
1.000
Potassium chloride
0.200
Magnesium sulphite
0.200
Bromocresol Purple
0.0075
Agar
15.400
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 22.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C,
mix well and pour into sterile petri plates. Ensure complete solidification and inoculate test
sample aseptically.
