For the isolation of Alicyclobacillus species in fruit juices.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber color clear to slightly opalescent
pH
4.0 ± 0.2 at 25˚C (29.00 g/ l)
Solidification
40˚C
Color
Light amber color clear to slightly opalescent gel
Contents
Ingredients
Gram/Litre
Yeast Extract
2.000
Glucose
5.000
Calcium chloride dihydrate
0.250
Magnesium sulfate heptahydrate
0.500
Ammonium sulfate
0.200
Potassium dihydrogen phosphate
3.000
Agar
18.000
pH at 25˚C
4.0 ± 0.2
Directions
Dissolve 29.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45°C,
and adjust the pH to 4.0 ± 0.2 with sterile 1N H2SO4. Mix well and pour into sterile petri plates.
Avoiding air bubbles while pouring. Ensure complete solidification and inoculate test sample
aseptically.
