For the isolation and presumptive identification of group D Streptococci.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light brownish beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light Amber colored opalescent
pH
6.6 ±0.2 at 25˚C (63.00 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Meat Extract
3.00
Peptone
5.00
Oxgall
40.00
Ferric Citrate
0.50
Esculin
1.00
Agar
15.00
pH at 25˚C
6.6 ±0.2
Directions
Dissolve 64.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min (Overheating may
causes darkening of medium), cool it to 42-45 °C and dispense in desired (Test tubes to form butt
and in petri plates for isolation and identification). Ensure complete solidification and inoculate
test sample aseptically.
