Bile Esculin Agar

ARTICLE NO. OLFC-345
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For the isolation and presumptive identification of group D Streptococci.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder.
Color Light brownish beige colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚C
Color Light Amber colored opalescent
pH 6.6 ±0.2 at 25˚C (63.00 g/ l)
Solidification 40˚C
Color Light amber colored opalescent gel

Contents

Ingredients Gram/Litre
Meat Extract3.00
Peptone5.00
Oxgall40.00
Ferric Citrate0.50
Esculin1.00
Agar15.00
pH at 25˚C6.6 ±0.2


Directions

Dissolve 64.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min (Overheating may causes darkening of medium), cool it to 42-45 °C and dispense in desired (Test tubes to form butt and in petri plates for isolation and identification). Ensure complete solidification and inoculate test sample aseptically.


Frequently Asked Questions

Where can I find the TDS for Bile Esculin Agar?
The Technical Data Sheet (TDS/IFU) and MSDS for Oxford Lab Fine Chem's Bile Esculin Agar (Article No. OLFC-345) are both available for direct download on the product page.
What is Bile Esculin Agar used for?
It is used for the isolation and presumptive identification of group D Streptococci in clinical and non-clinical microbiology testing.
What are the key ingredients in Bile Esculin Agar?
The formulation includes meat extract, peptone, oxgall, ferric citrate, esculin, and agar, prepared to a pH of 6.6 ± 0.2.
How is Bile Esculin Agar prepared?
Dissolve 64.50 grams in 1000 ml distilled water, boil to dissolve completely, sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes, cool to 42-45°C, then dispense into tubes or plates.
What pack sizes are available for Bile Esculin Agar?
It is available in 100 gm and 500 gm packs.