For propagation of pathogenic cocci and other fastidious organisms associated with blood
culture work and allied pathological investigations.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light yellow to slight amber colored
pH
7.4 ±0.2 at 25 ˚C (37.00 g/ l)
Contents
Ingredients
Gram/Litre
Beef Heart Infusion (From 250g)
5.000
Protease Peptone
10.000
Calf Brains Infusion (From 200g)
12.500
Dextrose
2.000
Sodium Chloride
5.000
Disodium Phosphate
2.500
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 37.00 grams in 1000 ml distilled. Boil to dissolve the medium completely and
sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate
test sample aseptically.
