For the selective isolation of Salmonella other than S.typhi from faeces, food and dairy
products.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Pinkish beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light orange brown colored opalescent
pH
6.9 ±0.2 at 25˚C (58.00 g/ l)
Solidification
40˚C
Color
Light orange brown colored opalescent gel
Contents
Ingredients
Gram/Litre
Proteose Peptone
10.000
Yeast Extract
3.000
Lactose
10.000
Saccharose
10.000
Sodium Chloride
5.000
Brilliant Green
0.012
Phenol Red
0.080
Agar
20.000
pH at 25˚C
6.9 ±0.2
Directions
Dissolve 58.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, (AVOID
OVERHEATING) cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete
solidification and inoculate test sample aseptically.
