For enumeration of coliform bacteria in water, sewage and foods.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Greenish beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Emerald green, clear solution
pH
7.2 ±0.2 at 25 ˚C (40.01 g/ l)
Contents
Ingredients
Gram/Litre
Peptone
8.250
Lactose
1.900
Sodium sulphite
0.205
Ferric chloride
0.0295
Monopotassium phosphate
0.0153
Erioglaucine
0.0649
Basic fuchsin
0.0776
Bile salt
0.00295
Brilliant Green
0.00003
Agar
10.150
pH at 25˚C
6.9 ±0.2
Directions
Dissolve 20.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, (AVOID
OVERHEATING) cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete
solidification and inoculate test sample aseptically.
