Recommendation for cell culture, cell growth, and viability research to support the growth.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige to light pink
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Reddish orange, clear solution
pH
7.40-8.00 at 25 ˚C (13.5g/ l)
Contents
Ingredients
Gram/Litre
INORGANIC SALTS
mg/l
Calcium chloride dihydrate
265.000
Ferric nitrate nonahydrate
0.100
Magnesium sulphate anhydrous
97.720
Potassium chloride
400.000
Sodium chloride
6400.000
Sodium dihydrogen phosphate anhydrous
109.000
AMINO ACIDS
mg/l
Glycine
30.000
L-Arginine hydrochloride
84.000
L-Cystine dihydrochloride
62.570
L-Glutamine
584.000
L-Histidine hydrochloride monohydrate
42.000
L-Isoleucine
105.000
L-Leucine
105.000
L-Lysine hydrochloride
146.000
L-Methionine
30.000
L-Phenylalanine
66.000
L-Serine
42.000
L-Threonine
95.000
L-Tryptophan
16.000
L-Tyrosine disodium salt
103.79
L-Valine
94.000
VITAMINS
mg/l
Choline chloride
4.000
D-Ca-Pantothenate
4.000
Nicotinamide
4.000
Pyridoxal hydrochloride
4.000
Riboflavin
0.400
Thiamine hydrochloride
4.000
i-Inositol
7.200
OTHERS
mg/l
D-Glucose
4500.000
Sodium pyruvate
110.000
Phenol red sodium salt
15.900
Directions
Dissolve 13.5 gram in 1000 ml distilled water mix well to dissolve all the ingredients. Adjust the pH 7.40-8.00 using 1N HCl/NaOH. Filter the medium through a sterile membrane filter (0.22μm). Aseptically dispense the medium in desired.
