For examination of fuels for microbial contamination and also for examine hydrocarbon
deterioration by bacteria.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Tan colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored with white precipitate.
pH
7.0 ±0.2 at 25 ˚C (23.27 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel with yellow orange precipitate
Contents
Ingredients
Gram/Litre
Magnesium Sulfate
0.200
Calcium Chloride
0.020
Monopotassium Phosphate
1.000
Ammonium Phosphate Dibasic
1.000
Potassium Nitrate
1.000
Ferric Chloride
0.050
Agar
20.000
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 23.27 grams in 1000 ml distilled water check. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
For hydrocarbon decomposing ability: Dissolve 23.27 grams in 1000 ml distilled water check and
adjust the pH if required so that after sterilization it is 7.0±0.2. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C
mix with sterile test hydrocarbon and distribute aseptically in petri plates. Ensure complete
solidification and inoculate test sample aseptically.
