For the isolation and cultivation of fastidious microorganisms like Neisseria gonorrhoeae.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light amber colored opalescent
pH
7.2 ±0.2 at 25˚C (45.50 g/ l)
Solidification
40 ˚C
Color
Chocolate brown colored opaque gel (with haemoglobin solution)
Contents
Ingredients
Gram/Litre
Protease Peptone
20.00
Dextrose
0.50
Sodium Chloride
5.00
Disodium hydrogen Phosphate
5.00
Agar
15.00
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 45.50 grams in 495 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C. Add 500 ml of sterile hemoglobin solution and mix thoroughly and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically. Sterile 2% hemoglobin solution: Add 10 ml of hemoglobin to distilled water and bring volume to 500 ml. Mix thoroughly and autoclave for 15 min at 15lbs pressure at 21°C. Cool at 45°C -50°C. The media can also be enriched with yeast autolysate supplement or vitamin growth supplement reconstituted. Mix well before pouring.
