For isolation and cultivation of coliform organisms from milk and milk products.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light Beige colored powder
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Purple color, clear slightly opalescent solution
pH
7.0 ±0.2 at 25 ˚C (16.00 g/ l)
Contents
Ingredients
Gram/Litre
Proteose peptone
10.000
Yeast Extract
6.000
Bile salts
20.000
Sodium deoxycholate
0.100
Lactose
20.000
Sodium lauryl sulphate
1.000
Bromocresol purple
0.035
pH at 25˚C
7.0 ±0.2
Directions
Dissolved 57.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically in test tubes containing inverted Durham’s tubes. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
