A semisynthetic medium used for general cultivation of fungi, yeasts and soil bacteria.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light amber colored opalescent
pH
7.3 ±0.2 at 25 ˚C (49.00 g/ l)
Solidification
40 ˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Sucrose
30.00
Sodium Nitrate
2.00
Dipotassium Phosphate
1.00
Magnesium Sulphate
0.50
Potassium Chloride
0.50
Ferrous Sulphate
0.01
Agar
15.00
pH at 25˚C
7.3 ±0.2
Directions
Dissolve 49.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically. Ensure complete solidification and inoculate test sample aseptically.
