Semisynthetic medium for cultivation of fungi capable of utilizing sodium nitrate as the sole source of nitrogen.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Off white colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light yellow colored, clear to slightly opalescent solution
pH
7.3 ±0.2 at 25 ˚C (35.00 g/ l)
Contents
Ingredients
Gram/Litre
Sucrose
30.00
Sodium Nitrate
3.00
Dipotassium Phosphate
1.00
Magnesium Sulphate
0.50
Potassium Chloride
0.50
Ferrous Sulphate
0.01
pH at 25˚C
7.3 ±0.2
Directions
Dissolve 35.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
