For the cultivation and maintenance of Aspergillus niger.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder
Solubility
Soluble in Distilled/de-ionized water at 50°C
Solidification
40˚C
Color
Light amber colored, slightly opalescent gel with precipitate
Contents
Ingredients
Gram/Litre
Sucrose
30.000
Yeast extract
5.000
Dipotassium hydrogen phosphate
1.000
Sodium nitrate
0.300
Potassium chloride
0.050
Magnesium sulfate
0.050
Ferrous sulfate
0.001
Zinc sulfate
0.001
Copper sulfate
0.0005
Agar
15.000
Directions
Dissolve 51.40 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45°C and distribute aseptically. Ensure complete solidification and inoculate test sample aseptically.