For isolation of iron bacteria, especially those from Sphaerotilus-Leptothris group.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light yellow to colorless opalescent gel
pH
5.1 ±0.2 at 25 ˚C (24.00 g/ l)
Solidification
40 ˚C
Contents
Ingredients
Gram/Litre
Dextrose
0.150
Ammonium sulphate
0.500
Calcium Nitrate
0.010
Dipotassium hydrogen phosphate
0.050
Magnesium sulphate
0.050
Potassium chloride
0.50
Calcium carbonate
0.100
Cyanocobalamin
0.00001
Thiamine
0.0004
Agar
10.000
Directions
Dissolve 11.00 grams in 1000 ml distilled water. Heat just to boil and transfer in desired. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
