For detection and enumeration of faecal coliforms by membrane filter technique at higher
temp.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light yellow to greyish yellow colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
After Addition of 1% Rosolic Acid: Red colored slightly opalescent solution
pH
7.4 ±0.2 at 25˚C (52.00 g/ l)
Solidification
40˚C
Color
Red colored slightly opalescent gel
Contents
Ingredients
Gram/Litre
Tryptose
10.000
Proteose Peptone
5.000
Yeast extract
3.000
Lactose
12.500
Bile salt mixture
1.500
Sodium chloride
5.000
Aniline blue
0.100
Agar
15.000
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 52.10 grams in 1000 ml distilled water containing 10 ml of 1% rosalic acid.
Boil to dissolve the medium completely. Do not autoclave, cool it to 42-45 °C and distribute
aseptically in sterile petri plates. Ensure complete solidification and place membrane filter
aseptically.
