For cultivation of lactic Streptococci and plaque assay of lactic bacteriophages.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige to tan colored
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light to medium amber colored, slightly opalescent gel
pH
7.1 ± 0.2 at 25˚C (33.25 g/ l)
Solidification
40˚C
Color
Light to medium amber colored, slightly opalescent
Contents
Ingredients
Gram/Litre
Peptone
5.000
Soya Peptone
5.000
Yeast Extract
2.500
Beef Extract
5.000
Lactose
5.000
Ascorbic acid
0.500
Magnesium sulphate
0.250
Agar
10.000
pH at 25˚C
7.1 ±0.2
Directions
Dissolve 33.25 grams in 1000 ml distilled water. Add 19 grams of Disodium
βGlycerophosphate. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs
pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure
complete solidification and inoculate test sample aseptically.
