For the presumptive identification of coliforms from water, milk and foods products.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Purple colored opalescent
pH
7.4 ±0.2 at 25˚C (70.00 g/ l)
Contents
Ingredients
Gram/Litre
Peptone
40.00
Lactose
20.00
Oxgall
10.00
Bromocresol Purple
0.02
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 70.00 grams in 1000 ml distilled water, boil to dissolve the medium completely and distribute in test tubes with inverted Durham tube. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
