For presumptive identification of coliforms from water, milk and foods products.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Purple, clear opalescent solution
pH
7.3 ±0.2 at 25 ˚C (35.00 g/ l)
Contents
Ingredients
Gram/Litre
Pancreatic Digest of Gelatin
20.000
Lactose Monohydrate
10.000
Dehydrated Ox-Bile
5.000
Bromocresol Purple
0.010
pH at 25˚C
7.3 ±0.2
Directions
Dissolve 35.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute in desire. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15
min, cool it to 42-45 °C and inoculate test sample aseptically.
