For the selective enrichment and enumeration of coliforms from water.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light pink colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Red colored opalescent clear solution
pH
7.4 ±0.2 at 25℃ (40.10 g/ l)
Contents
Ingredients
Gram/Litre
Peptone
20.000
Lactose
10.000
Bile Salt
5.000
Sodium Chloride
5.000
Neutral Red
0.070
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 40.10 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute in test tubes containing inverted Durham’s tube. Sterilize by
autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample
aseptically.
