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Fraser Broth Base

ARTICLE NO. OLFC-720
HSN NO. 38210000

MSDS

TDS(IFU)


Pack Size

Size
100 GM
500 GM

Application

Recommended selective, enrichment medium for the isolation of Listeria species.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Beige colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚ C
Color Light amber color, clear slightly opalescent solution
pH 7.2±0.2 at 25˚C (55.00 g/ l)
Prepared medium (with supplements) Color Pale-yellow color, clear slightly opalescent solution
Prepared medium (with supplements) pH 7.2 ±0.2 at 25 ˚C

Contents

Ingredients Gram/Litre
Peptone (Enzymatic digest of animal tissue)5.000
Tryptone (Enzymatic digest of casein)5.000
Yeast Extract5.000
Meat Extract5.000
Sodium Chloride20.000
Disodium hydrogen phosphate dihydrate9.600
Potassium dihydrogen phosphate1.350
Esculin1.000
Lithium Chloride3.000
pH at 25˚C7.2 ±0.2


Directions

Dissolved 55.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and add rehydrated contents of 1 vial Fraser selective supplement and 2 vials of Fraser supplement. Mix well and distribute in sterile test tube and inoculate test sample aseptically.