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Fraser Broth Base, Modified (Half Fraser Broth)

ARTICLE NO. OLFC-722
HSN NO. 38210000

MSDS

TDS(IFU)


Pack Size

Size
100 GM
500 GM

Application

For the selective enrichment of Listeria species from food products.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Beige colored powder.
Solubility Soluble in Distilled/de-ionized water at 50°C
Color Light amber color, clear slightly opalescent solution
pH 7.2±0.2 at 25˚C (55.00 g/ l)

Contents

Ingredients Gram/Litre
Peptone5.000
Tryptone5.000
Yeast Extract5.000
Meat Extract5.000
Sodium Chloride20.000
Disodium Hydrogen Phosphate9.600
Potassium Dihydrogen Phosphate1.350
Esculin1.000
Lithium Chloride3.000
Nalidixic acid0.010
Acriflavine0.012
pH at 25˚C7.2 ±0.2


Directions

Dissolved 55.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and add rehydrated contents of 2 vials of Fraser supplement (250mg Ferric ammonium citrate). Mix well and distribute in sterile test tube and inoculate test sample aseptically.