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Fraser Secondary Enrichment Broth Base

ARTICLE NO. OLFC-725
HSN NO. 38210000

MSDS

TDS(IFU)


Pack Size

Size
100 GM
500 GM

Application

For isolation, cultivation and enrichment of Listeria monocytogenes from foods and other environmental samples.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Beige colored powder.
Solubility Soluble in Distilled/de-ionized water at 50°C
Color Light amber color, clear slightly opalescent solution
pH 7.2±0.2 at 25˚C (57.85 g/ l)
Prepared medium (with supplements) Color Pale-yellow color, clear slightly opalescent solution
Prepared medium (with supplements) pH 7.2 ±0.2 at 25˚C

Contents

Ingredients Gram/Litre
Proteose Peptone5.000
Tryptone5.000
Yeast Extract5.000
Meat Extract5.000
Sodium Chloride20.000
Disodium Hydrogen Phosphate12.000
Potassium Dihydrogen Phosphate1.350
Esculin1.000
Lithium Chloride3.000
Ferric ammonium citrate0.500
pH at 25˚C7.2 ±0.2


Directions

Dissolved 57.85 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and add rehydrated contents of 2 vials of Fraser Selective supplement. Mix well and distribute in sterile test tube and inoculate test sample aseptically.