Deoxycholate Citrate Agar

ARTICLE NO. OLFC-615
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For the selective isolation and cultivation of enteric pathogens such as Salmonella & Shigella species.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Pinkish beige colored powder.
Solubility Soluble in Distilled/de-ionized water at 50°C
Color Orange red colored opalescent
pH 7.3 ±0.2 at 25 ˚C (55.50 g/ l)
Solidification 40 ˚C
Color Orange red colored opalescent gel

Contents

Ingredients Gram/Litre
Meat extract4.550
Proteose Peptone4.550
Lactose9.090
Sodium Deoxycholate0.450
Ferric Ammonium Citrate0.900
Sodium Citrate7.720
Sodium thiosulphate7.720
Neutral Red0.023
Agar20.450
pH at 25˚C7.3 ±0.2


Directions

Dissolve 55.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely. DO NOT STERILIZE BY AUTOCLAVING, cool it to 42-45 °C and distribute aseptically in to the petri plates. Ensure complete solidification and inoculate test sample aseptically.