For disinfectant testing, wherein neutralization agent is important for determining its bactericidal activity.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Bluish beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Purple to reddish colored opalescent
pH
7.6 ±0.2 at 25 ˚C (54.00 g/ l)
Solidification
40 ˚C
Color
Purple to reddish colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
5.00
Yeast Extract
2.50
Dextrose
10.00
Sodium Thioglycolate
1.00
Sodium Thiosulphate
6.00
Sodium Bisulfate
2.50
Polysorbate 80
5.00
Lecithin
7.00
Bromocresol purple
0.02
Agar
15.00
pH at 25˚C
7.6 ±0.2
Directions
Dissolve 54.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically in desired, allow to solidify and inoculate the test sample.
