For antibiotic sensitivity testing of fastidious pathogens like Neisseria, Streptococcus & Haemophillus species with blood enrichment.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Pale yellow colored opalescent solution
pH
7.4 ±0.2 at 25℃ (43.04 g/ l)
Solidification
40 ˚C
Color
Pale yellow colored opalescent gel
Contents
Ingredients
Gram/Litre
Proteose peptone
10.000
Veal Infusion solids
10.000
Dextrose
2.000
Sodium chloride
3.000
Disodium hydrogen phosphate
2.000
Sodium acetate
1.000
Adenine sulphate
0.010
Guanine hydrochloride
0.010
Uracil
0.010
Xanthine
0.010
Thiamin
0.00002
Agar
15.0
pH at 25˚C
7.4±0.2
Directions
Dissolve 43.04 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in desired. Ensure complete solidification and inoculate test sample aseptically.
