For the primary isolation of coliforms from large samples such as water or waste water.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light pink colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Red colored, clear solution
pH
7.5 ±0.2 at 25 ˚C (80.00 g/ l)
Contents
Ingredients
Gram/Litre
Peptone
40.000
Lactose
20.000
Bile Salt
10.000
Sodium Chloride
10.000
Neutral Red
0.140
pH at 25˚C
7.5 ±0.2
Directions
Dissolve 80.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute in test tubes containing inverted Durham’s tube. Sterilize by
autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample
aseptically.
