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Middlebrook 7H9 Agar Base

ARTICLE NO. OLFC-1127
HSN NO. 38210000

MSDS

TDS(IFU)


Pack Size

Size
100 GM
500 GM

Application

For cultivation and sensitivity testing of Mycobacterium tuberculosis.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Light yellow to light green powder
Prepared Medium (Color) Light amber colored clear to slightly opalescent gel with a greenish tinge in petri dishes
pH 6.60 ± 0.2 at 25˚C

Contents

Ingredients Gram/Litre
Ammonium sulphate0.500
Sodium glutamate0.500
Sodium citrate0.100
Pyridoxine0.001
Biotin0.0005
Disodium hydrogen phosphate2.500
Potassium dihydrogen phosphate1.000
Ferric ammonium citrate0.040
Magnesium sulphate0.050
Calcium chloride anhydrous0.0005
Zinc sulphate0.001
Copper sulphate0.001
Malachite green0.001
Agar15.000
pH at 25°C6.6±0.2


Directions

Suspend 9.85 grams in 450 ml purified/distilled water. 1ml glycerol may be added if desired. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add I vial of Middlebrook OADC Growth Supplement (FD018). Mix well and distribute as desired.