For isolation of Streptococci from mixed cultures, especially Streptococcus mitis,
Streptococcus salivarius & Enterococcus faecalis.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Bluish beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Deep Blue colored opalescent
pH
7.0 ±0.2 at 25 ˚C (90.08 g/ l)
Solidification
40˚C
Color
Deep blue colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptose
10.000
Proteose Peptone
10.000
Dextrose
1.000
Sucrose
50.000
Dipotassium Phosphate
4.000
Trypan Blue
0.075
Crystal Violet
0.0008
Agar
15.000
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 90.08 grams of dehydrated media in 999 ml distilled water. Boil to dissolve
the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it
to 42-45 °C and add 1ml of 1% potassium tellurite solution (not provided with medium) mix well
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
