Modified Semisolid Rappaport Vassiliadis Medium Base

ARTICLE NO. OLFC-1149
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For the detection of motile Salmonella species from food, faeces and environmental specimens.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder.
Color Pale yellow with blue ting colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚ C
Color Greenish blue colored opalescent solution
pH 5.10-5.40 at 25˚C (39.47 g/ l)

Contents

Ingredients Gram/Litre
Enzymatic digest of animal and plant tissue4.600
Acid hydrolysate of casein4.600
Sodium Chloride7.300
Potassium dihydrogen phosphate (KH2PO4)1.500
Magnesium chloride hexahydrate (MgCl2.6H2O)40.000
Malachite green oxalate0.040
Agar2.700
pH at 25˚C5.1-5.40


Directions

Dissolve 39.47 grams in 1000 ml distilled water, boil to dissolve all ingredients by continuous stirring. Do not autoclave, cool to 40-45 ℃ add selective agent such as Novobiocin 10 mg/ 1000 ml and distribute in test tubes/ Petri plates allow to cool and inoculate test sample aseptically.