For the differentiation of bacteria based on their ability to decarboxylate the amino acid.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Purple colored clear solution
pH
6.0 ±0.2 at 25 ˚C (10.52 g/ l)
Contents
Ingredients
Gram/Litre
Peptone
5.000
Special peptone
5.000
Dextrose
0.500
Bromocresol purple
0.010
Cresol Purple
0.005
Pyridoxal
0.005
pH at 25˚C
6.0 ±0.2
Directions
Dissolve 10.50 grams in 1000 ml distilled water. Add 10 gm. of L-Lysine, L
Arginine, LOrnithine or other L-amino acids. When using DL-amino acids, use 2%
concentration (When L-Ornithine is added, adjust the pH to 6.0). Heat if necessary, to dissolve
the medium completely. Distribute 5.0 ml in screw-capped tubes and sterilize by autoclaving at
15 lbs pressure (121 °C) for 10 min, Cool to 20-25°C and aseptically inoculate test sample.
