For detection of Escherichia coli in water and food by fluorogenic method.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent solution
pH
6.9 ±0.2 at 25 ˚C (37.05 g/ l)
Contents
Ingredients
Gram/Litre
Tryptone
20.000
Lactose
5.000
Bile Salts
1.500
Dipotassium Phosphate
4.000
Potassium Dihydrogen Phosphate
1.500
Sodium Chloride
5.000
4-Methylumbelliferyl ß-D-Glucuronide (MUG)
0.050
pH at 25˚C
6.9 ±0.2
Directions
Dissolve 37.05 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute in test tube containing inverted Durham’s tube. Sterilize by
autoclaving at 15 lbs pressure (121 °C) for 12 min, cool it to 42-45 °C and inoculate test sample
aseptically.
