For differentiation of Streptococcus mutans & Streptococcus sanguinis associated with
oral microflora.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored solution
pH
7.3 ±0.2 at 25 ˚C (98.10 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Casein Enzymic Hydrolysate
15.00
Yeast Extract
5.00
L-Cystine
0.20
Sodium Sulphite
0.10
Sodium Chloride
1.00
Disodium Phosphate
0.80
Sodium Bicarbonate
2.00
Sodium Acetate
12.00
Sucrose
50.00
Agar
12.00
pH at 25˚C
7.3 ±0.2
Directions
Dissolve 98.10 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
