For detection of nitrate reduction and enumeration of Bacillus cereus.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored
pH
7.0 ±0.2 at 25˚C (9.00 g/ l)
Contents
Ingredients
Gram/Litre
Peptone
5.00
Meat Extract
3.00
Potassium Nitrate
1.00
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 9.0 grams in 1000 ml distilled water, boil to dissolve the medium
completely and distribute aseptically. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15
min, cool it to 42-45 °C and inoculate test sample aseptically. After incubation of 24 hrs. add few
drops of 0.8% sulfanilic acid and α-naphthylamine (0.5 g in 100 ml DW) to each tube. Observe
for development of a distinct red or pink color within 1-2 minutes, indicating reduction of nitrate
to nitrite.
