General culture medium, can be used as enriched medium with blood or other biological
fluids,
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Pale yellow colored opalescent solution
pH
7.4 ±0.2 at 25℃ (35.00 g/ l)
Solidification
40˚C
Color
Pale yellow colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
10.0
Meat Extract
10.0
Sodium Chloride
5.0
pH at 25˚C
7.5 ±0.2
Directions
Dissolve 25.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute aseptically. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15
min, cool it to 42-45 °C and inoculate test sample aseptically.