For the general cultivation of less fastidious microorganisms, can be enriched with blood.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored, clear to slightly opalescent solution.
pH
7.4 ±0.2 at 25 ˚C (13.00 g/ l)
Contents
Ingredients
Gram/Litre
Veg Peptone
5.00
Sodium Chloride
5.00
Veg Extract
1.50
Yeast Extract
1.50
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 13.00 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs.
pressure (121 °C) for 15 min, cool it to 42-45 °C. If desired, the medium can be enriched with 5
10% blood or other biological fluids.
