For differentiation of gram negative bacteria on the basis of fermentative and oxidative
metabolism of carbohydrates.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige with green yellow ting colored
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber, opalescent solution
pH
6.80 ±0.2 at 25˚C (9.38 g/ l)
Solidification
40˚C
Color
Green color, clear opalescent semi solid gel
Contents
Ingredients
Gram/Litre
Tryptone
2.000
Sodium chloride
5.000
Dipotassium phosphate
0.300
Bromothymol blue
0.080
Agar
2.000
pH at 25˚C
6.8 ±0.2
Directions
Dissolve 9.38 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs
pressure (121°C) for 15 minutes. Cool to 45-50°C and add 10 ml of 10% sterile carbohydrate
solution (10% Dextrose, 10% lactose or 10% Saccharose solution). Mix well and aseptically
transfer 5 ml in sterile test tube to form stab. Inoculate test sample aseptically.
