For differentiation of Enterobacteriaceae in urine, water and food samples.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light yellowish to creamy colored opalescent
pH
7.1 ±0.2 at 25 ˚C (20.88 g/ l)
Solidification
40˚C
Color
Blue colored, clear opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
2.000
Yeast Extract
1.000
Glucose
10.000
Sodium Chloride
5.000
Dipotassium Phosphate
0.300
Bromothymol Blue
0.080
Agar
2.500
pH at 25˚C
7.1 ±0.2
Directions
Dissolve 20.88 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C
and distribute aseptically in desired. Allow to cool at room temperature and inoculate test
sample aseptically.
