An agar-based medium for cultivating and isolating Streptomyces species.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Pale yellow colored opalescent solution
pH
7.2±0.2 at 25℃ (40.00 g/ l)
Solidification
40˚C
Color
Pale yellow colored opalescent gel
Contents
Ingredients
Gram/Litre
Glucose
4.000
Yeast extract
4.000
Malt extract
10.000
Calcium carbonate (CaCO3)
2.000
Agar
20.000
Directions
Dissolve 40.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in desired. Ensure complete solidification and inoculate test sample
aseptically.
